Virus Magnetic DNA Extraction Kit
This kit uses unique separation role of magnetic beads and unique
buffer fluid system, from GMF plant organization separation and
purification of high quality genomic DNA, special coated beads,
under certain conditions on the target DNA has a strong affinity,
When conditions change, the beads release of absorbed DNA, to
achieve the purpose of rapid separation and purification of DNA,
the whole process does not involve toxic reagents, safe,
convenient, extracted genomic DNA of high purity, stable quality.
Use this kit purified DNA suitable for a variety of normal
operations, including Enzyme cut, PCR library construction,
Southern hybridization experiments.
Plant Genomic DNA Extraction Kit
Animal Genomic DNA Extraction Kit
Whole Blood Magnetic DNA Extraction Kit
Forensic samples Genomic DNA Extraction Kit
Rapid method plasmid DNA Extraction Kit
Virus Magnetic DNA Extraction Kit
1. Simple and fast: It can usually obtain ultra-pure genomic DNA in
2. A wide range of applicable:
Crops Genomic Magnetic DNA Extraction Kit: for fresh plant leaves,
stems and seeds, embryos.
Herbal Medicine Genomic Magnetic DNA Extraction Kit: for some
pre-treated plants (such as high temperature or the DNA serious
GMF Magnetic DNA Extraction Kit: for the DNA extraction of
transgenic species and processed foods.
Marine Organism Genomes Magnetic DNA Extraction Kit: for animal
tissues, especially the marine life.
Whole Blood Genomic DNA Extraction Kit: for small doses of the
blood extraction, especially in dry blood.
Rapid method plasmid DNA Extraction Kit: for rapid extraction of
high purity plasmid DNA from bacterial culture solution.
Bone genomic DNA Extraction Kit: for DNA extraction from bone,
teeth, hair and other more rigid material.
3. It can ensure high purity without washing magnetic beads many
times. The typical value of the extracted genomic DNA OD260/OD280
can be 1.7-2.0
GMF Magnetic DNA Extraction Kit
30ml * 1 bottle
1ml * 2 bottles
Magnetic beads mixing liquid
25ml * 1 bottle
50ml * 1 bottle
15ml * 1 bottle
1. Sample preparation Take sample 25mg-100mg, torn into small
pieces into the mortar for grinding
2. Lysis: using miniature hand drill carefully drill a clean spongy
bone about 0.2 grams, added to the 1.5ml centrifuge tube (EP tube)
by adding 300μL lysis buffer and bufferA40μL, oscillating uniform,
65-degree temperature bath 60 minutes.
3. Combining: removed the EP tube from the warm bath device,
12000r/min centrifugation for 10 minutes, transferred 200μL
supernatant carefully to a new EP tube, add mixing magnetic beads
liquid with 250μL, 5-point vibration mixing, place in room
temperature for 10 minutes, in which the EP tube upside down mixing
every 2-3 minutes. After 10 minutes magnetic separation, put the EP
tube on the magnet rack for magnetic adsorption, this time the
magnetic beads were adsorbed on the wall, then please use a smaller
tip suction waste fluid, to avoid siphoning off the beads.
4 Washing: Add 250μL washing liquid, mix gently point vibration,
for 10 seconds, and then magnetic separation, sucked clean residual
liquid of pipe and the bottom of tube, then add 250μL washing
liquid second sucked clean residual liquid of pipe and the bottom
of tube, dry for 5 minutes at room temperature for openings.
5. Eluting: Add 60-100μL elution, slow suction 6 times blending,
and placed at room temperature for 10 or 15 minutes, gently rocking
EP tubes every 2-3 minutes. Then magnetic separation, please be
careful to draw the clear liquid turn to the new centrifuge tube
for downstream experiments.